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Image Search Results
Journal: PLoS ONE
Article Title: MiR-486 Regulates Lactation and Targets the PTEN Gene in Cow Mammary Glands
doi: 10.1371/journal.pone.0118284
Figure Lengend Snippet: MiR-486 targets PTEN directly as measured by luciferase reporter assays, pGL3-wt: pGL3/PTEN and pGL3-mu: pGL3/PTEN/mut; pGL3-control: the empty vector, Luciferase activities of pGL3-wt are markedly decreased in cells transfected with miR-486 compared to those of reporter plasmids with mut or empty vectors. The data are the averages of at least three independent runs, and the values are the means±SD. * P <0.05 compared to the negative control.
Article Snippet: HEK-293 cells were cultured in 24-well plates, and each plate was transfected with 80 ng of
Techniques: Luciferase, Plasmid Preparation, Transfection, Negative Control